Journal: International Journal of Stem Cells

Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator

doi: 10.15283/ijsc25040

Figure Lengend Snippet: Characterization of pluripotent stem cell markers in iPSCs. (A) Immunofluorescence staining showing qualitative expression of pluripotency markers SOX2 and TRA-1-60 in iPSC cell lines N7, N9, N11, and N12. Scale bar=200 μm. (B) qRT-PCR analysis of gene expression levels of pluripotency markers NANOG and OCT4 in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). (C) Immunofluorescence staining showing qualitative expression of the hematopoietic commitment marker IGF2 in the iPSC cell lines. Scale bar=200 μm. (D) qRT-PCR analysis of gene expression levels of the hematopoietic commitment marker IGF2 and its receptor IGF1R in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). iPSCs: induced pluripotent stem cells, IGF2: insulin-like growth factor II.

Article Snippet: The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).

Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression, Marker

Journal: Translational Neurodegeneration

Article Title: Transplantation of hiPSC-derived pericytes rescues Alzheimer’s disease phenotypes in APOE 4/4 mice through IGF2-rich apoptotic vesicles

doi: 10.1186/s40035-025-00512-6

Figure Lengend Snippet: IGF2 in ApoVs plays a vital role in functional recovery of APOE 4/4-PCs in vitro. a ApoVs derived from APOE 3/3-PCs or HDFs were subjected to transcriptomic analysis and proteomic analysis. b Principal component analysis (PCA) evaluating the similarities of gene expression profiles between ApoVs −HDFs and ApoVs −PCs . c, d Volcano plot and heatmap of differential gene expression analysis between ApoVs −HDFs and ApoVs −PCs . e – h The differentially expressed genes were subjected to GO enrichment analysis. The GO terms related to neuron protection and cognition ( e ), blood–brain barrier maintenance ( f ), DNA replication and transcription ( g ) and cell growth and metabolic ( h ) were significantly enriched in ApoVs −PCs . i, j Volcano plot ( i ) and heatmap ( j ) of protein expression between ApoVs −HDFs and ApoVs −PCs . k, l qPCR and western blot analysis of IGF2 mRNA expression and protein level in ApoVs −HDFs and ApoVs −PCs . m, n IGF2 knockout in APOE 3/3-PCs using the CRISPR-Cas9 system. IGF2 expression was confirmed by western blot analysis. o, p Flow cytometry analysis with annexin V and PI staining revealed cell death rate in APOE 3/3-PCs, APOE 4/4-PCs, APOE 4/4-PCs + ApoVs −PCs−IGF2KO and APOE 4/4-PCs + ApoVs −PCs−IGF2NC groups ( n = 3 biological repeats for each group). q Representative dextran leakage in APOE 3/3-PCs, APOE 4/4-PCs, APOE 4/4-PCs + ApoVs −PCs−IGF2KO and APOE 4/4-PCs + ApoVs −PCs−IGF2NC groups ( n = 3 biological repeats for each group). r Representative Aβ transcytosis in APOE 3/3-PCs, APOE 4/4-PCs, APOE 4/4-PCs + ApoVs −PCs−IGF2KO and APOE 4/4-PCs + ApoVs −PCs−IGF2NC groups ( n = 3 biological repeats for each group). One-way ANOVA with Tukey’s multiple comparison test; ns, non-significant, * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The primary antibodies were anti-Aβ 40 (Servicebio, Cat. #GB111197-100, 1:1000), Aβ1-42 Rabbit mAb (ABclonal, Cat. #A24422, 1:1000), phospho-tau-T181 rabbit mAb (ABclonal, Cat. #AP1387, 1:1000, Wuhan, China), and IGF2 (Signalway Antibody, Cat. #32592-2, 1:1000, Frederick, MA).

Techniques: Functional Assay, In Vitro, Derivative Assay, Gene Expression, Expressing, Western Blot, Knock-Out, CRISPR, Flow Cytometry, Staining, Comparison